Samuel married Elizabeth Keziah Douglas on 16 Nov 1847 in Enfield, Connecticut, USA. Elizabeth was born on 8 Oct 1828 in Littlefield, Maine, USA; died on 11 Jul 1856 in Westfield, Massachusetts, USA. [Group Sheet] [Family Chart]

1. Byron Allen Cowles was born on 18 Aug 1848 in Westfield, Massachusetts, USA; died after 1878 in Camp Verde, Arizona, USA.
2. Herbert Eugene Cowles was born on 15 Nov 1852 in Westfield, Massachusetts, USA; died in 1862 in McConnelsville, Ohio, USA.
3. Frederick J Cowles was born on 26 Jan 1856 in Westfield, Massachusetts, USA; died on 16 Apr 1856 in Westfield, Massachusetts, USA.

Samuel married Jane Emerson on 25 Apr 1858. Jane was born in Guernsey, Ohio, USA; died after 1894 in McConnellsville, Ohio, USA. [Group Sheet] [Family Chart]

1. Jessie Emerson Cowles was born on 9 Nov 1859 in McConnellsville, Ohio, USA; died in Urbana, Ohio, USA.
2. Jennie Lourine Cowles was born on 6 Nov 1863 in McConnellsville, Ohio, USA; died after 1894 in Nobel, Ohio, USA.