Samuel married Eliza Douglas on 25 May 1829 in Oakland County, Michigan. Eliza (daughter of David Douglas and Rhoda Phelps) was born on 10 Sep 1809 in Whitestown, Oneida County, New York; died on 19 Dec 1868 in Fayette County, Iowa. [Group Sheet] [Family Chart]

1. William Colby was born on 14 Oct 1830 in Pontiac, Oakland County, Michigan; died on 2 Aug 1916.
2. George Colby was born on 17 Oct 1832 in Pontiac, Oakland County, Michigan; died on 17 Jan 1917.
3. James H. Colby was born on 20 Apr 1834 in Pontiac, Oakland County, Michigan; died on 5 Nov 1905.
4. David Colby was born on 28 Nov 1835 in Pontiac, Oakland County, Michigan; died on 12 Dec 1916.
5. Mary Colby was born on 12 Jun 1838 in Pontiac, Oakland County, Michigan; died on 2 Mar 1841 in Pontiac, Oakland County, Michigan.
6. Melissa Colby was born on 19 Oct 1840 in Pontiac, Oakland County, Michigan; died on 28 Aug 1913 in Oregon City, Clackamas County, Oregon.
7. Samuel F. Colby was born on 18 Aug 1843 in Rock County, Wisconsin; died on 19 Oct 1893 in Spring Grove, Des Moines County, Iowa.